GMHI: Gut Microbiome Health Index

Related tags

MiscellaneousGMHI
Overview

GMHI: Gut Microbiome Health Index

Anaconda-Server Badge Anaconda-Server Badge Anaconda-Server Badge Anaconda-Server Badge Anaconda-Server Badge

Description

Gut Microbiome Health Index (GMHI) is a robust index for evaluating health status based on the species-level taxonomic profile of a stool shotgun metagenome (gut microbiome) sample.

Installation

To avoid dependency conflicts, create an isolated conda environment and install gmhi

  1. Install via conda
conda create --name gmhi_env -c danielchang2002 python=3.7 gmhi
  1. Activate environment
conda activate gmhi_env

Usage

usage: gmhi [-h] [-o OUTPUT] --fastq1 FASTQ1 --fastq2 FASTQ2

DESCRIPTION:
GMHI version 1.0
Gut Microbiome Health Index (GMHI) is a robust index for evaluating 
health status based on the species-level taxonomic profile of a stool 
shotgun metagenome (gut microbiome) sample.

AUTHORS:
Daniel Chang, Vinod Gupta, Jaeyun Sung

USAGE:
GMHI is a pipeline that takes as input two raw fastq files generated 
from a paired end sequence, performs quality control, estimates microbial 
abundances, and returns as output a health index score.

* Profiling a metagenome from raw reads:
$ gmhi --fastq1 metagenome1.fastq --fastq2 metagenome2.fastq

optional arguments:
  -h, --help            show this help message and exit
  -o OUTPUT, --output OUTPUT
                        Output file name

required named arguments:
  --fastq1 FASTQ1       first input fastq file
  --fastq2 FASTQ2       second input fastq file

Example

Directory structure:

.
├── metagenome1.fastq
└── metagenome2.fastq

Command:

gmhi --fastq1 metagenome1.fastq --fastq2 metagenome2.fastq -o GMHI2.txt

Result:

.
├── GMHI.txt
├── abundance.txt
├── metagenome1.fastq
└── metagenome2.fastq

where GMHI2.txt is a text file with a single line containing the health index score of the metagenome, and abundance.txt is a tsv containing the estimated microbial abundances.

Runtime

Runtime depends on the size of the input metagenome and the system specs.

On a 2019 MacBook Pro with a 2.3 GHz 8-Core Intel Core i9 processor and 16GB of RAM, a single run of gmhi on an input metagenome of 4 GB takes 29 minutes.

Note: the initial run on any machine will take extra time because databases will need to be downloaded and installed before the actual computation.

Owner
Daniel Chang
Daniel Chang
NewsBlur is a personal news reader bringing people together to talk about the world.

NewsBlur NewsBlur is a personal news reader bringing people together to talk about the world.

Samuel Clay 6.2k Dec 29, 2022
Decoupled Smoothing in Probabilistic Soft Logic

Decoupled Smoothing in Probabilistic Soft Logic Experiments for "Decoupled Smoothing in Probabilistic Soft Logic". Probabilistic Soft Logic Probabilis

Kushal Shingote 1 Feb 08, 2022
Yet another Python Implementation of the Elo rating system.

Python Implementation - Elo Rating System Yet another Python Implementation of the Elo rating system (how innovative am I right?). Only supports 1vs1

Kraktoos 5 Dec 22, 2022
Application launcher and environment management

Application launcher and environment management for 21st century games and digital post-production, built with bleeding-rez and Qt.py News Date Releas

10 Nov 03, 2022
This is a a CSMA/CA simulator written in Python based on simulator of the same type

This is a a CSMA/CA simulator written in Python based on simulator of the same type found the link https://github.com/StevenSLXie/CSMA-Simulator with

M. Ismail 4 Nov 22, 2022
Discovering local read-level DNA methylation patterns and DNA methylation heterogeneity in intermediately methylated regions

Discovering local read-level DNA methylation patterns and DNA methylation heterogeneity in intermediately methylated regions

1 Jan 11, 2022
JPMC Virtual Experience

This repository contains the submitted patch files along with raw files of the various tasks assigned by JPMorgan Chase & Co. through its Software Engineering Virtual Experience Program on Forage (fo

Vardhini K 1 Dec 05, 2021
This is a Blender 2.9 script for importing mixamo Models to Godot-3

Mixamo-To-Godot This is a Blender 2.9 script for importing mixamo Models to Godot-3 The script does the following things Imports the mixamo models fro

8 Sep 02, 2022
A program that lets you use your tablet's tilting to emulate an actual joystick on a Linux computer.

Tablet Tilt Joystick A program that lets you use your tablet's tilting to emulate an actual joystick on a Linux computer. It's called tablet tilt joys

1 Feb 07, 2022
Automatically load and dump your dataclasses 📂🙋

file dataclasses Installation By default, filedataclasses comes with support for JSON files only. To support other formats like YAML and TOML, filedat

Alon 1 Dec 30, 2021
Very simple encoding scheme that will encode data as a series of OwOs or UwUs.

OwO Encoder Very simple encoding scheme that will encode data as a series of OwOs or UwUs. The encoder is a simple state machine. Still needs a decode

1 Nov 15, 2021
Zeus is an open source flight intellingence tool which supports more than 13,000+ airlines and 250+ countries.

Zeus Zeus is an open source flight intellingence tool which supports more than 13,000+ airlines and 250+ countries. Any flight worldwide, at your fing

DeVickey 1 Oct 22, 2021
Online-update est un programme python permettant de mettre a jour des dossier et de fichier depuis une adresse web.

Démarrage rapide Online-update est un programme python permettant de mettre a jour des dossier et de fichier depuis une adresse web. Mode préconfiguré

pf4 2 Nov 26, 2021
A way to write regex with objects instead of strings.

Py Idiomatic Regex (AKA iregex) Documentation Available Here An easier way to write regex in Python using OOP instead of strings. Makes the code much

Ryan Peach 18 Nov 15, 2021
Easy to use phishing tool with 65 website templates. Author is not responsible for any misuse.

PyPhisher [+] Description : Ultimate phishing tool in python. Includes popular websites like facebook, twitter, instagram, github, reddit, gmail and m

KasRoudra 1.1k Dec 31, 2022
jonny is a stack based programming language

jonny-lang jonny is a stack based programming language also compiling jonny files currently doesnt work on windows you can probably compile jonny file

1 Nov 24, 2021
A web application which you can search, buy or sell shares with current prices which provided by IEX.

CS50 - Stock Exchange A web application which you can search, buy or sell shares with current prices which provided by IEX. Table of Contents Setup St

1 May 28, 2022
Distribute PySPI jobs across a PBS cluster

Distribute PySPI jobs across a PBS cluster This repository contains scripts for distributing PySPI jobs across a PBS-type cluster. Each job will conta

Oliver Cliff 1 Feb 10, 2022
Paprika is a python library that reduces boilerplate. Heavily inspired by Project Lombok.

Image courtesy of Anna Quaglia (Photographer) Paprika Paprika is a python library that reduces boilerplate. It is heavily inspired by Project Lombok.

Rayan Hatout 55 Dec 26, 2022
Adds a Bake node to Blender's shader node system

Bake to Target This Blender Addon adds a new shader node type capable of reducing the texture-bake step to a single button press. Please note that thi

Thomas 8 Oct 04, 2022