[Space & single cell omics] phase 2: combined single cell and bulk transcriptome to identify two epithelial tumor cell states in colorectal cancer, and improved CMS typing

This document sharing ,dy The title of the literature explained is ： Combine single cells and bulk Transcriptome identifies two epithelial tumor cell states in colorectal cancer , And perfected CMS parting . (Single-cell and bulk transcriptome sequencing identifies two epithelial tumor cell states and refines the consensus molecular classification of colorectal cancer)

I read No 2 When you go all over the place , Only then can I understand the innovation of this article , In fact, he conceived a New typing . And I think this idea should be applied to other cancers .

The video has been synchronized to B standing , You can move B Stand and learn (UP Lord ：TOP bacteria ).

Colorectal cancer CMS Typing is based on bulk Transcriptome , Very classic , However, the potential diversity of epithelial cells is still unclear . author Integrated analysis From 63 One patient 、189 Samples 、37 Million single cell data , Finally, the focus is close 5 Ten thousand epithelial cells .

And then , be based on 「 gene expression 、 Copy number variation 、 Gene regulatory networks 」 And so on , The tumor cells were divided into two groups . These two kinds of tumor Cell intrinsic subtype , stay 3,614 Of bulk All samples can be verified , Then these bulk The samples were classified as 「iCMS2 and iCMS3」.

iCMS3 Of patients , Further divided into MSI-H( Microsatellite instability ) and MSS( Microsatellite stability ) Two groups , and iCMS2 Most of them are MSS Of . Tradition CMS In typing CMS4( A group with obvious matrix characteristics , stay CMS Of 4 The prognosis is poor in the three groups ) stay iCMS2 and iCMS3 You can see it in the new classification , Be divided into CMS4 and iCMS3 Of patients have the worst prognosis .

Author combination Epithelial intrinsic subgroup (I), Microsatellite instability (M)、 Fibroblastic characteristics (F) Three aspects of information , Put forward "IMF" parting , Divide the patients into 5 Group , The characteristics of each group are as follows ：

The article is of great significance , It's worth reading a few more times ~