[Space & single cell omics] phase 2: single cell map of intratumoral changes in patients with breast cancer after anti-pd1 treatment

This week's literature sharing ,jw I explained a single-cell literature related to treatment , The content is ： Patients with breast cancer go through anti-PD1 After treatment , Single cell map of changes in tumor .

The video has been synchronized to B standing ： The explanations were very detailed , You can move B Stand and learn .

In combination with the following jw The explanation and the original content , A brief introduction

background

Previous studies have shown that , After receiving immunotherapy for breast cancer , On the whole, we can get some relief and prolong survival . For all that , There are still some people who did not respond .

Are there any potential mechanisms or markers that can predict treatment response , It's very important , This is also the purpose of this article .PD1、PD-L1、 Lymphocyte infiltration 、TMB Wait, these are all known markers , Besides T Cell cloning and amplification can also indicate ICB Treatment response of . This article is also a topic launched from this starting point .

Experimental design

take anti-PD1 Paired samples before and after treatment , At the same time scRNA-seq and scTCR-seq. according to T The clonal type of cells divides patients into clonal amplification (E) And no clonal amplification (NE) Two groups .

result

Many results of the article are before treatment / After treatment ,E / NE Such groups are compared . The author will also have activation 、 Characteristic of exhaustion T The cell is named Tex cells(experienced T cells). Such cells have proliferative characteristics , expression PD1 molecular , And E Group ratio NE Group higher . Judging from the proportion of cells , These cells are E There are also more groups .

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These data show that ： expression PD1 Of T Percentage of cells and T Cell clonal expansion is related , stay anti-PD1 After treatment ,CD8+ and CD4+ Of Tex Cells are the main amplification T cells .

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Pseudo temporal analysis found , stay naive T towards CD8+ Tex The pathway of cell transformation is late , come from E Of the group Tex Cell ratio NE More groups . CD4 T Cells performed a similar analysis , Found in naive T towards CD4 Tfh as well as CD4 Th1 The pathway of cell transformation is late , come from E Cell ratio of group NE More groups .

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Combine the above two contents , The author thinks that CD4 Tfh and CD4 Th1 Support CD8+ Tex The function of

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Then I explored , Is there a signature Can be used to predict T Clonal amplification of cells .

Yes DC Cell analysis showed ,DC Cells have the characteristics of immune response , Can support T The function of cells .

Then on macrophages 、 Tumor cells are analyzed ...

Then cell communication analysis ...

Summary and discussion

• The subject has been systematically evaluated by ICB After treatment , Cellular and molecular changes in the tumor of patients with breast cancer ;
• Patients with breast cancer are receiving anti-PD1 After treatment , Are there any markers that can be predicted T Cell clone amplification ？ The author in pre-treatment Immune microenvironment , Found some features .

That's great , I just listened 3 All the time ~