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Error: grouping factors must have ＞ 1 sampled level

2022-07-17 22:59:00 【Analysis of breeding data】

Report errors ：
Error: grouping factors must have > 1 sampled level

Error code ：

Analyze more than one year's data , It is necessary to consider the location and the area group within the location , Both use random factors of mixed linear models .

> ##  modeling 
> blup <- lmer(First~(1|Line)+(1|Loc)+(1|Rep%in%Loc)+(1|Line:Loc), data=phe)
Error: grouping factors must have > 1 sampled level

here %in% Replace with /.

The correct code is as follows ：

> ##  modeling 
> blup <- lmer(First~(1|Line)+(1|Loc)+(1|Loc/Rep)+(1|Line:Loc), data=phe)
> summary(blup)

Variance component results ：

That's it ！

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